Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Por um escritor misterioso
Descrição
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2–16 hours, limiting its potential in applications like rapid diagnostics. We present here a set of conditions for RNA FISH (dubbed Turbo RNA FISH) that allow us to make accurate measurements with no more than 5 minutes of hybridization time and 3 minutes of washing, and show that hybridization times can go as low as 30 seconds while still producing quantifiable images. We further show that rapid hybridization is compatible with our recently developed iceFISH and SNP FISH variants of RNA FISH that enable chromosome and single base discrimination, respectively. Our method is simple and cost effective, and has the potential to dramatically increase the throughput and realm of applicability of RNA FISH.
Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers
A technical review and guide to RNA fluorescence in situ hybridization [PeerJ]
SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues
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Viral detection and identification in 20 minutes by rapid single-particle fluorescence in-situ hybridization of viral RNA
Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Figure 6 from Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Single-molecule FISH in Drosophila muscle reveals location dependent mRNA composition of megaRNPs
Methods for spatial and temporal imaging of the different steps involved in RNA processing at single‐molecule resolution - Markey - 2021 - WIREs RNA - Wiley Online Library
Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
High-fidelity amplified FISH for the detection and allelic discrimination of single mRNA molecules
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RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH).
RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH).
RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH).
