Expediting Antibody Discovery with a Cell and Bead Multiplexed
Por um escritor misterioso
Descrição
A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.
Expediting Antibody Discovery with a Cell and Bead Multiplexed
One Stop Solution for Antibody Drug Discovery
Using Multiplexed Screening for Advanced Therapeutic Antibody
High-Powered Multiplexing with Flow Cytometry in High-Throughput
Luminex Discovery Assays R&D Systems, a Bio-Techne Brand
Using Multiplexed Screening for Advanced Therapeutic Antibody
Our focus is to accelerate biomarker research to enable better
Multiplex Immunoassays in the COVID-19 Vaccine Race
One Stop Solution for Antibody Drug Discovery